Gas chromatographic mass spectroscopic evaluation of Punarnavadi Kwatha

Abstract

Punarnavadi kwatha is a most commonly used polyherbal formulation in Ayurvedic medical practice. It is also called as Punarnavashtaka kwatham. For the purpose of quality assurance and uniformity, scientific verification of Ayurvedic medications is important. Usage of modern analytical techniques helps in the proper authentication of medicines. Gas chromatographic mass spectroscopic evaluation is one among the modern analytical tool to assess the bio molecules in the formulation. Punarnavadi kwatha was prepared according to the classical method and Gas Chromatographic Mass Spectroscopic evaluation was done using DB5MS column. 27 compounds were identified in the formulation. The compound 1,2,3-Benzenetriol was identified with the maximum percentage area 17.189%. On comparing the phytoconstituents of individual ingredient drugs, showed the presence of chemical compounds from ingredients. From the analysis the specific compounds from ingredients can be used as a marker compound so that we can analyse the presence of all ingredients in the polyherbal formulation. So, gas chromatographic mass spectroscopic evaluation can be used as a tool in quality control and standardisation.